Chloroquine transfection mechanism

Discussion in 'Canada Drugs' started by ChaoS, 04-Mar-2020.

  1. Chloroquine transfection mechanism


    Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short term studies of protein function. We specifically use this protocol with Lenti-X 293T cells, a cell line optimized for production of lentiviral vectors.

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    Used as a transfection enhancement reagent, the mechanisms by which CQ enhances gene expression remain unclear. CQ is a weak base with pKas of 8.1 and 10.215 and is known to buffer the luminal pH of endosomes.16,17 The buffering activity of CQ could improve transfection efficiency by facilitating DNA release from the endocytic pathway as it may Chloroquine has been frequently used in cell culture gene transfection studies Cotton et al. 1992, Wagner et al. 1992, Lucas et al. 1995, but has not been reported yet to be successful for enabling gene transfection in vivo. As mentioned above, the mechanisms of action of chloroquine seems to be related to elevating the intravesicular pH. Similarly, the transformation frequency of rat cells following polyoma DNA transfection was approximately 6-fold increased by chloroquine treatment. The effect of the compound was even more pronounced in transfections with linear forms of polyoma DNA, suggesting that chloroquine inhibits degradation of DNA absorbed by the cells.

    Last Upload: June 10, 2016 Day 0: Seed Lenti-X 293T cells (this cell line is optimized for production of lentiviral vectors) Day 1 (pm): Transfect Cells Day 2 (am): 18h post transfection - Remove media, replace with fresh media Day 3 or more (am): Observe fluorescence, harvest cells, or perform your experiment *Pro-Tips* Different brands and lots of FBS can promote or inhibit transfection. This approach can be adapted for different cell lines and different transfection reagents.

    Chloroquine transfection mechanism

    Chloroquine diphosphate salt powder or crystals, 98.5-101.0% dry basis., Enhanced plasmid DNA transfection with lysosomotropic agents in.

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  5. Article On the Mechanism of Chloroquine Resistance. daily without chloroquine is not as abundant as a single one-time collection between 48-60 hours after initial transfection with chloroquine.

    • Questions with answers in CHLOROQUINE Science topic.
    • High efficiency polyoma DNA transfection of chloroquine treated cells..
    • Addgene General Transfection.

    Chloroquine has been used to increase transfection efficiency for calcium phosphate, DEAE-dextran, and some liposome-mediated gene delivery 63,65–68. However, effects of lysosomotropic agents on polymer and some lipid based methods of gene delivery are still controversial in the literature. Chloroquine is an inhibitor of the lysosomal degradation of the DNA which is taken up by the cells, so as leelee said, transfection should have been successful, albeit at a slightly lower level than if you would have added the chloroquine. In my normal transfections, I never add chloroquine and get high efficiencies nevertheless. In gene transfection 21. TheamountofCQinpolyplexesinthe delivery of anti-miR-210 into the cancer cells, was associated with the efficient miRNA delivery as higher content of chloroquine caused increased endosomal escape 22. In another study con-ducted by Yang Hu et al. in the presence of 75 μM concentration

     
  6. nata_blin User

    Take this medicine exactly as directed by your doctor. Treatments for Rheumatoid Arthritis Chloroquine Aralen - Side Effects, Dosage, Interactions - Drugs Chloroquine MedlinePlus Drug Information
     
  7. demion New Member

    Hydroxychloroquine Plaquenil Side Effects & Dosage for Malaria Hydroxychloroquine Plaquenil is a drug that is classified as an anti-malarial drug. Plaquenil is prescribed for the treatment or prevention of malaria. It is also prescribed for the treatment of rheumatoid arthritis, lupus, and the side effects of lupus such as hair loss, joint pain, and more.

    Plaquenil hydroxychloroquine 200mg is it causing itchy skin.
     
  8. Chat Noir Well-Known Member

    My Take on New Ocular Screening Guidelines for Plaquenil. The study findings revealed that when hydroxychloroquine was used at doses 5mg /kg, the risk of retinopathy was 1% in the first 5 yrs. 2% in 10 yrs. 20 % after 20 yrs. There was a dose response, such that those receiving doses greater that 5 mg / kg had the greatest risk of retinal toxicity.

    New App to Tackle Hydroxychloroquine Dosing Dilemma.