Chloroquine and p62

Discussion in 'Canada Drugs Online' started by Sebastano, 02-Mar-2020.

  1. mchost_ru XenForo Moderator

    Chloroquine and p62


    Chloroquine has been extensively used in mass drug administrations, which may have contributed to the emergence and spread of resistance. It is recommended to check if chloroquine is still effective in the region prior to using it.

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    Conversely, chloroquine-treated VCPR155H/+ mice revealed progressive muscle weakness, cytoplasmic accumulation of TDP-43, ubiquitin-positive inclusion bodies and increased LC3-I/II, p62/SQSTM1, and optineurin expression levels. Our in vitro patient myoblasts studies treated with rapamycin demonstrated an overall improvement in the autophagy. Figure 1 Assesment of functional basal autophagy in vitro. A Immunoblotting of LC3B and p62 upon pharmacological autophagy inhibition. A dose-dependent increase of LC3B and p62 was observed with increasing concentrations of chloroquine. Immunoprecipitation of SQSTM1/p62 protein from PANC-1 cell extracts. Lane 1 is 10% input, lane 2 is Mouse G3A1 mAb IgG1 Isotype Control #5415, and lane 3 is SQSTM1/p62 D5L7G Mouse mAb. Western blot was performed using SQSTM1/p62 D5L7G Mouse mAb. A light chain-specific secondary antibody was used to avoid reactivity with heavy chain IgG.

    The Centers for Disease Control and Prevention recommend against treatment of malaria with chloroquine alone due to more effective combinations. In areas where resistance is present, other antimalarials, such as mefloquine or atovaquone, may be used instead.

    Chloroquine and p62

    Premo™ Autophagy Sensor GFP-p62 Kit, Expression analysis of LC3B and p62 indicates intact.

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  6. Check your blood sugar as you have been told by your doctor. A very bad and sometimes deadly reaction has happened with chloroquine. Most of the time, this reaction has signs like fever, rash, or swollen glands with problems in body organs like the liver, kidney, blood, heart, muscles and joints, or lungs.

    • Chloroquine Indications, Side Effects, Warnings -.
    • CST - SQSTM1/p62 D5L7G Mouse mAb.
    • Akt and Autophagy Cooperate to Promote Survival of Drug..

    The expression of the autophagy markers LC3B and p62 were assessed via immunofluorescence and immunoblotting after pharmacological autophagy inhibition with chloroquine, which prevents the fusion of autophagosomes and lysosomes by increasing lysosomal pH, in HT-29 and LoVo CC cell lines. In addition to genetic means, autophagy can be disabled by the lysosomal inhibitor chloroquine or by bortezomib, which blocks autophagy via a cathepsin-dependent mechanism resulting in up-regulation of p62. p62 is rich in protein-interacting sequences and contains domains that include a protein-protein interaction module PB1 that facilitates its oligomerization, a ubiquitin-associated UBA 3 domain, and an LC3 interaction region that are essential for p62 function 8, 13, 14. Moreover, elevated p62 is significantly correlated with poor survival in breast cancer patients Supplementary Figure 1, suggesting a role for autophagy in breast cancer reoccurrence 14-18. HCQ is a lysotropic chloroquine derivative that accumulates within lysosomes, resulting in lysosome neutralization and the inhibition of autophagic flux.

     
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